Beverage-Air NC49 manuales

Beverage air NC49 Manual (67 paginas)

Marca: Beverage-air | Categoria: No | Tamaño: 2.31 MB |

Inglés

Tabla de contenidos

AminoPac

1

AAA-Direct

1

©Dionex Corporation, 2006

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Document No. 031481

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Revision 12

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November 2006

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TABLE OF CONTENTS

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SECTION 1 - INTRODUCTION

7

2.1 System Requirements

10

3.1 Deionized Water

12

3.2 Sodium Hydroxide

12

3.3 Sodium Acetate

12

SECTION 4 - GETTING STARTED

13

4.2 Initial Check List

14

5.5 Amino Acid Standards

16

6.1.2 Software Installation

18

6.1.3 System Rinse

18

6.2.1 System Background Check

18

Ca. 30 nC

19

6.4.2 Column Installation

20

6.4.3 Histidine Injection

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Overlay of lines 3 and 4

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Peak height ~150 nC

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6.6 System Shutdown

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Figure 5

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Overview of Gradient Methods

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SECTION 8 - APPLICATIONS

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HCl Hydrolysate

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M Standard

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8.3 Analysis of Tryptophan

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Standard

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Performic Acid/HCl

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Hydrolysate

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0 5.00 10.00 15.00 20.00

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Carrot Juice

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Figure 16B

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Figure 16A

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8.10 AAA Recommended Reading

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Eluent 1: 10 mM NaOH

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Eluent 2: 250 mM NaOH

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Gradient Conditions for

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above gradient table

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SECTION 10 - TROUBLESHOOTING

49

10.3 Column Problems

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10.4 System Problems

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Oxygen Dip

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10.5 Sodium Hydroxide Cleanup

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10.6 Nitric Acid Cleanup

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10.7 Acetate Line Cleanup

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10.8.1 Mechanical Polishing

56

Reference Electrode

63

Cylinder

63

Thumbscrew

63

Cell Cover

63

Cell Body

63

J2 Connector

64

Junction

65

APPENDIX A - COLUMN CARE

66

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